Home > Sandwich ELISA Kits > Mouse Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) ELISA Kit

Product Name:

Mouse Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) ELISA Kit

Cat.#:

JL15705

Regulatory Status:

RUO

Alternative:

MAC-2-BP; LGALS3-BP; Galectin-3-Binding Protein; L3 Antigen; Mac-2-Binding Protein; Tumor-associated antigen 90K; Serum Protein 90K; Basement membrane autoantigen p105

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.12ng/mL

Specificity:

This kit recognizes Mouse LGALS3BP in samples. No significant cross-reactivity or interference between Mouse LGALS3BP and analogues was observed.

Sample Type:

Serum, plasma and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| LGALS3BP

Galectin-3-binding protein is a protein thatis encoded by the LGALS3BP gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. Using fluorescence in–situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds to galectin 1.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.08	1.58	1.06	0.7	0.43	0.3	0.23	0.08
Corrected OD	2	1.5	0.98	0.62	0.35	0.22	0.15	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse LGALS3BP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	87-101	96
Plasma(n=8)	92-105	101

| Linearity

Samples were spiked with high concentrations of Mouse LGALS3BP and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	88-103	87-107
1 : 4	Average (%)	94	98